Article
MethylQuant: a real-time PCR-based method to quantify DNA methylation at single specific cytosines.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Dugast-Darzacq Claire, Grange Thierry
Abstract excerpt
MethylQuant is a cost-effective and relatively simple technique which enables quantitative analysis of the methylation status of a single cytosine at specific positions in DNA that can be assimilated to the quantitative detection of a single nucleotide polymorphism (SNP). After bisulfite conversion of DNA and PCR amplification of the region of interest, the methylation status is quantified by methylation-specific...
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