Article

A novel imaging biosensor for the detection of reversed replication forks and four-way junctions in human cells

2026-08-24

Abstract excerpt

Replication fork reversal and recombination-dependent replication protect perturbed forks by forming four-way DNA junctions. Despite their central role, detecting these intermediates in intact cells has historically required electron microscopy of bulk extracted DNA. Here, we describe a genetically encoded biosensor for four-way junctions based on nuclear-targeted bacterial RuvA tagged with GFP or Spot-tag. Combin...

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Identifiers and source

Literature Corpus work: 2445909a-6965-5a28-abf3-c79fc972a644
DOI: 10.64898/2026.08.23.746510

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