Article
Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
2020-04-14
Abstract excerpt
The CRISPR/Cas12a RNA-guided complexes have a tremendous potential for nucleic acid detection due to its ability to indiscriminately cleave ssDNA once bound to a target DNA. However, the current CRISPR/Cas12a systems are limited to detecting DNA in a picomolar detection limit without an amplification step. Here, we developed a platform with engineered crRNAs and optimized conditions that enabled us to detect DNA,...
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Identifiers and source
- Literature Corpus work
- 23e9508b-6343-5bc0-8d53-2229a450822e
- DOI
- 10.1101/2020.04.13.036079