Article

Structural dynamics of DNA strand break sensing by PARP-1 at a single-molecule level

2022-02-25

Abstract excerpt

<title>Abstract</title> <p>Single stranded breaks (SSBs) are the most frequent DNA lesions threatening genomic integrity. A highly kinked DNA structure in complex with human PARP-1 led to the proposal that SSB sensing in Eukaryotes relies on dynamics of both the broken DNA double helix and PARP-1’s multi-domain organization. Here, we directly probe this fundamental yet poorly understood process at the single-mole...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 20c0ff3f-1549-5a42-bd68-1ea7844fe695
DOI: 10.21203/rs.3.rs-1345876/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Structural dynamics of DNA strand break sensing by PARP-1 at a single-molecule levelDOI 10.21203/rs.3.rs-1345876/v1Read

Structural dynamics of DNA strand break sensing by PARP-1 at a single-molecule levelSemantic proximity Read

Structural dynamics of DNA strand break sensing by PARP-1 at a single-molecule levelSemantic proximity Read

Structural Basis of Detection and Signaling of DNA Single-Strand Breaks by Human PARP-1Semantic proximity Read

A novel approach reveals genomic landscapes of single-strand DNA breaks with nucleotide resolution in human cellsSemantic proximity Read

The DNA-binding domain of human PARP-1 interacts with DNA single-strand breaks as a monomer through its second zinc finger.Semantic proximity Read

Protein-mediated stabilization and nicking of the non-template DNA strand dramatically affect R-loop formation <i>in vitro</i>Semantic proximity Read

PARP-1 Activation Requires Local Unfolding of an Autoinhibitory Domain.Semantic proximity Read

Single-molecule force spectroscopy reveals binding and bridging dynamics of PARP1 and PARP2 at DNA double-strand breaksSemantic proximity Read

A PARP2-specific active site α-helix melts to permit DNA damage-induced enzymatic activationSemantic proximity Read

Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistanceSemantic proximity Read

Novel DNA endonuclease activity of human ERK1 proteinSemantic proximity Read

Lesion search and recognition by thymine DNA glycosylase revealed by single molecule imaging.Semantic proximity Read