Article

Structural dynamics of DNA strand break sensing by PARP-1 at a single-molecule level

2022-02-26

Abstract excerpt

Single stranded breaks (SSBs) are the most frequent DNA lesions threatening genomic integrity. A highly kinked DNA structure in complex with human PARP-1 led to the proposal that SSB sensing in Eukaryotes relies on dynamics of both the broken DNA double helix and PARP-1's multi-domain organization. Here, we directly probe this fundamental yet poorly understood process at the single-molecule level. Quantitative SM-...

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Literature Corpus work: 2c47593b-4c6a-52ad-9ac9-e9143eddac02
DOI: 10.1101/2022.02.26.482089

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