Article

Efficient Zygotic Genome Editing via RAD51-Enhanced Interhomolog Repair

2018-02-11

Abstract excerpt

Recent advances in genome editing have greatly improved knock-in (KI) efficiency 1–9 . Searching for factors to further improve KI efficiency for therapeutic use and generation of non-human primate (NHP) models, we found that the strand exchange protein RAD51 can significantly increase homozygous KI using CRISPR/Cas9 in mouse embryos through an interhomolog repair (IHR) mechanism. IHR is well-described in the con...

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Literature Corpus work: 1f9d1820-984d-546a-8325-591f007184fa
DOI: 10.1101/263699

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