Article

Donor-Free Gene Correction by Targeted Interhomolog Recombination

2019-02-03

Abstract excerpt

Spontaneous gene correction by interhomolog recombination (IHR) occasionally occurs to ameliorate genetic diseases of blood and skin 1–3 . Using an engineered endogenous gene as a reporter, we demonstrate that gene correction by IHR is normally infrequent (≤0.02%) but is stimulated by DSBs targeted by CRISPR/Cas9 to both homologous chromosomes; reaching frequencies of 0.5%. We further show that depletion of POLQ...

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Literature Corpus work: 4c104b49-10c9-56d7-bee8-9aef81109e9f
DOI: 10.1101/538603

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