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Article

Efficient embryonic homozygous gene conversion via RAD51-enhanced interhomolog repair.

Cell - 10 Jun 2021

Wilde Jonathan J, Aida Tomomi, Del Rosario Ricardo C H, Kaiser Tobias, Qi Peimin, Wienisch Martin, Zhang Qiangge, Colvin Steven, Feng Guoping

Abstract excerpt

Searching for factors to improve knockin efficiency for therapeutic applications, biotechnology, and generation of non-human primate models of disease, we found that the strand exchange protein RAD51 can significantly increase Cas9-mediated homozygous knockin in mouse embryos through an interhomolog repair (IHR) mechanism. IHR is a hallmark of meiosis but only occurs at low frequencies in somatic cells, and its...

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Share to Chromosomes, Mammalian
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Breaks, Double-Stranded

Share to Gene Conversion

Share to Homologous Recombination

Share to Rad51 Recombinase

Share to Ribonucleoproteins

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Source

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Identifiers

PMID: 34043941
DOI: 10.1016/j.cell.2021.04.035
PMCID: PMC8240950

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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