Article

Harnessing endogenous repair mechanisms for targeted gene knock-in of bovine embryos

2020-06-12

Abstract excerpt

<h4>ABSTRACT</h4> Introducing useful traits into livestock breeding programs through gene knock-ins has proven challenging. Typically, targeted insertions have been performed in cell lines, followed by somatic cell nuclear transfer cloning, which can be inefficient. An alternative is to introduce genome editing reagents and a homologous recombination (HR) donor template into embryos to trigger homology-directed r...

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Literature Corpus work: ee2bde18-d382-53ec-8c06-5b2ab9b5f7aa
DOI: 10.1101/2020.06.09.143115

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