Article

REpair of heterozygous Mutations independent of Exogenous Donor template with high efficiency (REMEDY) using allele specific CRISPR targeting and HDR enhancers

2025-05-30

Abstract excerpt

<h4>Summary</h4> We report the development of REMEDY (REpair of heterozygous Mutations independent of Exogenous Donor template with high efficiencY), a genome editing strategy that allows efficient repair of heterozygous mutations in human and mouse cells without necessitating an exogenous donor DNA template. Here, we used in-PAM or near-PAM CRISPR strategies to induce a double-strand break (DSB) in mutant allele...

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Literature Corpus work: 6031ab5e-c078-57f0-90fe-ab16b4ae0bfd
DOI: 10.1101/2025.05.29.641201

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REpair of heterozygous Mutations independent of Exogenous Donor template with high efficiency (REMEDY) using allele specific CRISPR targeting and HDR enhancersDOI 10.1101/2025.05.29.641201Read

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