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Cryo-EM structure of cell-free synthesized human histamine H 2 receptor coupled to heterotrimeric G s protein in lipid nanodisc environment

2023-07-27

Abstract excerpt

Here we describe the cryo-electron microscopy structure of the human histamine 2 receptor (H 2 R) in an active conformation with bound histamine and in complex with G s heterotrimeric protein at an overall resolution of 3.4 Å. The complex was generated by cotranslational insertion into preformed nanodisc membranes using cell-free synthesis in E. coli lysates. It is the first structure obtained by this detergent...

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Literature Corpus work: 1dc9a916-d5fb-5dd3-866c-e16cfb28b80c
DOI: 10.1101/2023.07.27.550782

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Cryo-EM structure of cell-free synthesized human histamine H 2 receptor coupled to heterotrimeric G s protein in lipid nanodisc environmentDOI 10.1101/2023.07.27.550782Read

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Cryo-EM structure of cell-free synthesized human histamine H <sub>2</sub> receptor coupled to heterotrimeric G <sub>s</sub> protein in lipid nanodisc environment

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