Generation of human knock-in organoids by CRISPR-HOT

Abstract excerpt

<title>Abstract</title> <p>This protocol outlines reagents and procedures to generate human knock-in organoids using our established strategy named CRISPR-HOT. The protocol describes the plasmids to be used and strategies to generate knock-in organoids from different organs.</p>

Topics

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• CRISPR-Associated Protein 9
• Deoxyribonuclease I
• DNA End-Joining Repair
• Electroporation
• Gene Knock-In Techniques
• Organoids
• Recombinational DNA Repair
• Staining and Labeling

Identifiers and source

Literature Corpus work

689378d4-5301-5416-8831-c608fcd444be

DOI

10.21203/rs.3.pex-791/v1