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Article

A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells.

International journal of molecular sciences - 17 Jun 2023

Cuevas-Ocaña Sara, Yang Jin Ye, Aushev Magomet, Schlossmacher George, Bear Christine E, Hannan Nicholas R F, Perkins Neil D, Rossant Janet, Wong Amy P, Gray Michael A

Abstract excerpt

Introducing or correcting disease-causing mutations through genome editing in human pluripotent stem cells (hPSCs) followed by tissue-specific differentiation provide sustainable models of multiorgan diseases, such as cystic fibrosis (CF). However, low editing efficiency resulting in extended cell culture periods and the use of specialised equipment for fluorescence activated cell sorting (FACS) make hPSC genome...

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Identifiers

PMID: 37373413
DOI: 10.3390/ijms241210266
PMCID: PMC10299534

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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