Article

Halfpipe: a tool for analyzing metabolic labeling RNA-seq data to quantify RNA half-lives

2024-09-22

Abstract excerpt

We introduce Halfpipe, a tool for analyzing RNA-seq data from metabolic RNA labeling experiments. Its main features are the absolute quantification of 4sU-labeling-induced T>C conversions in the data as generated by SLAM-seq, calculating the proportion of newly synthesized transcripts, and estimating subcellular RNA half-lives. Halfpipe excels at correcting critical biases caused by typically low labeling efficien...

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Literature Corpus work: 15ea2417-21c8-563a-bb56-fe6fe54b8fa6
DOI: 10.1101/2024.09.19.613510

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