Article

Single Nucleotide Resolution 4sU Sequencing (SNU-Seq) reveals the transcriptional responsiveness of an epigenetically primed human genome

2021-07-14

Abstract excerpt

Genomes are pervasively transcribed, leading to stable and unstable transcripts that influence 3-dimensional genome organisation and gene regulation. High sensitivity and nucleotide resolution are required to resolve mammalian nascent transcriptomes. Here, we exploit the sensitivity of 4-thio-uridine (4sU) metabolic pulse-labelling to develop two nucleotide-resolution methods: S ingle- N ucleotide resolution 4s...

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Literature Corpus work: 2a985daf-1031-5b32-b4ed-34ada5876e11
DOI: 10.1101/2021.07.14.452379

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Single Nucleotide Resolution 4sU Sequencing (SNU-Seq) reveals the transcriptional responsiveness of an epigenetically primed human genomeDOI 10.1101/2021.07.14.452379Read

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