Article

Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells

2019-07-11

Abstract excerpt

Human pluripotent stem cells are increasingly used for CRISPR-mediated gene targeting in efforts to generate models of human diseases. This is a challenging task because of the high sensitivity of these cells to suboptimal conditions, including CRISPR-associated DNA damage and subsequent rounds of single-cell cloning. We sought to develop a sensitive method that enables rapid screening of CRISPR targeted cells, wh...

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Literature Corpus work: 15dca04d-cfdb-5a1c-a403-fb2426d47c8c
DOI: 10.12688/wellcomeopenres.15119.3

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