Article

A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications

2025-06-18

Abstract excerpt

T7 RNA polymerase is ubiquitously used in the fields of synthetic biology and biotechnology. Yet the ability to precisely and modularly regulate T7 RNAP remains surprisingly limited. Here, we developed a T7 RNAP regulatory toolbox consisting of programmable synthetic repressors, activators, and biosensors in a cell-free system. This toolbox enables the scalable design of T7 RNAP based gene regulatory networks and...

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Literature Corpus work: dbd1cf95-8479-524d-94ba-d60e3860c48a
DOI: 10.1101/2025.06.18.660355

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