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Article

Sensitive analysis of single nucleotide variation by Cas13d orthologs, EsCas13d and RspCas13d.

Biotechnology and bioengineering - 1 Aug 2021

Qiao Xin, Gao Yanmin, Li Jiaojiao, Wang Zhaoguan, Qiao Hongyan, Qi Hao

Abstract excerpt

RNA-guided CRISPR (RNA-targeting clustered regularly interspaced short palindromic repeats) effector Cas13d is the smallest Class II subtype VI proteins identified so far. Here, two recently identified Cas13d effectors from Eubacterium siraeum (Es) and Ruminococcus sp. (Rsp) were characterized and applied for sensitive nucleic acid detection. We demonstrated that the special target triggered collateral cleavage...

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PMID: 33964175
DOI: 10.1002/bit.27813

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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