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Directed evolution of <i>Anabaena variabilis</i> phenylalanine ammonia-lyase (PAL) identifies mutants with enhanced activities

2020-02-23

Abstract excerpt

There is broad interest in engineering phenylalanine ammonia-lyase (PAL) for its biocatalytic applications in industry (fine-chemicals and natural product synthesis) and medicine (phenylketoruria/PKU and cancer treatment). While site-specific mutagenesis has been employed to improve PAL stability or substrate specificity, a more comprehensive mutational landscape has yet to be explored for this class of enzymes. H...

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Identifiers and source

Literature Corpus work
0ae5d40e-5d99-5d1c-b063-b15edfd3e6dc
DOI
10.1101/2020.02.20.933945
Open publication

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