Article

Sequential Amino Acid Mutagenesis-Driven De Novo Evolution of Adenine Deaminases Enables Efficient In Vivo Base Editing in Primate

2025-05-16

Abstract excerpt

Base editing allows for the precise modification of genetic information, providing new avenues for treating diseases 1 . The adenine base editor ABE8e is currently the most efficient and widely used tool for adenine base editing. ABE8e was developed through multiple rounds of directed evolution of Escherichia coli tRNA adenine deaminase, including phage-assisted continuous evolution (PACE) 2,3 . While PACE is hi...

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Literature Corpus work: 3a1588e3-4b38-5ed6-a874-d26637fdf03a
DOI: 10.1101/2025.05.14.653640

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