Article
Enzyme engineering by targeted libraries.
Methods in enzymology - 1 Jan 2013
Goldsmith Moshe, Tawfik Dan S
Abstract excerpt
This review outlines the strategies we apply for directed enzyme evolution using targeted libraries, namely, libraries that diversify specific residues with predefined mutational compositions. The theoretical grounds underlining the design of such libraries are described, including the mutational load, the ratio of beneficial versus deleterious mutations, and screening capacity. We point out the advantage of...
Topics
- Directed Molecular Evolution
- Enzymes
- Gene Library
- Mutation
- Phylogeny
- Protein Engineering