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Article

Metabolic engineering of a genetic selection system with tunable stringency.

Proceedings of the National Academy of Sciences of the United States of America - 28 Aug 2007

Kleeb Andreas C, Edalat Maryam Hansson, Gamper Marianne, Haugstetter Johannes, Giger Lars, Neuenschwander Martin, Kast Peter, Hilvert Donald

Abstract excerpt

The biosynthesis of small molecules can be fine-tuned by (re)engineering metabolic flux within cells. We have adapted this approach to optimize an in vivo selection system for the conversion of prephenate to phenylpyruvate, a key step in the production of the essential aromatic amino acid phenylalanine. Careful control of prephenate concentration in a bacterial host lacking prephenate dehydratase, achieved...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 17715291
DOI: 10.1073/pnas.0705379104
PMCID: PMC1955800

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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