Article
Selecting better biocatalysts by complementing recoded bacteria
2022-08-16
Abstract excerpt
In vivo selections are powerful tools for the directed evolution of enzymes. However, the need to link enzymatic activity to cellular survival makes selections for enzymes that do not fulfill a metabolic function challenging. Here, we present an in vivo selection strategy that leverages recoded organisms addicted to non-canonical amino acids (ncAAs) to evolve biocatalysts that can provide these building blocks fro...
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Identifiers and source
- Literature Corpus work
- ca65a055-3022-5553-ad32-3b22442047be
- DOI
- 10.1101/2022.08.16.504095