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Phenylalanine ammonia-lyases: combining protein engineering and natural diversity.

Applied microbiology and biotechnology - 1 Feb 2023

Tomoiagă Raluca Bianca, Tork Souad Diana, Filip Alina, Nagy Levente Csaba, Bencze László Csaba

Abstract excerpt

In this study, rational design and saturation mutagenesis efforts for engineering phenylalanine ammonia-lyase from Petroselinum crispum (PcPAL) provided tailored PALs active towards challenging, highly valuable di-substituted substrates, such as the L-DOPA precursor 3,4-dimethoxy-L-phenylalanine or the 3-bromo-4-methoxy-phenylalanine. The rational design approach and saturation mutagenesis strategy unveiled...

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PMID: 36662259
DOI: 10.1007/s00253-023-12374-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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