Thermodynamically programmed one-pot CRISPR platform for point-of-care SNP genotyping

Abstract excerpt

<title>Abstract</title> <p>One-pot CRISPR diagnostics face a fundamental incompatibility: nucleic acid amplification requires rapid target accumulation, whereas CRISPR activation irreversibly consumes those substrates, destabilizing reaction kinetics. Existing strategies rely on empirical parameter balancing or external staging but lack an intrinsic mechanism to enforce reaction order within a single reactor. Her...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Cas Systems
• DNA Primers
• Genotyping Techniques
• Nucleic Acid Amplification Techniques
• Point-of-Care Systems
• Polymorphism, Single Nucleotide
• Thermodynamics

Identifiers and source

Literature Corpus work

8f4773c9-4c2f-5ec7-8707-afea0680f95b

DOI

10.21203/rs.3.rs-8802024/v1