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Article

Nuclease-mediated depletion biases in ribosome footprint profiling libraries

5 Jun 2020

Abstract excerpt

Ribosome footprint profiling is a high-throughput sequencing-based technique that provides detailed and global views of translation in living cells. An essential part of this technology is removal of unwanted, normally very abundant, ribosomal RNA sequences that dominate libraries and increase sequencing costs. The most effective commercial solution (Ribo-Zero) has been discontinued as a standalone product and a...

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PMID: 32503920
DOI: 10.1261/rna.075523.120

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