Article
High-content imaging-based pooled CRISPR screens in mammalian cells.
The Journal of cell biology - 1 Feb 2021
Yan Xiaowei, Stuurman Nico, Ribeiro Susana A, Tanenbaum Marvin E, Horlbeck Max A, Liem Christina R, Jost Marco, Weissman Jonathan S, Vale Ronald D
Abstract excerpt
CRISPR (clustered regularly interspaced short palindromic repeats)-based gene inactivation provides a powerful means for linking genes to particular cellular phenotypes. CRISPR-based screening typically uses large genomic pools of single guide RNAs (sgRNAs). However, this approach is limited to phenotypes that can be enriched by chemical selection or FACS sorting. Here, we developed a microscopy-based approach,...
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