Article

ITRAP2, a flexible and robust strategy to assign antigen recognition of T-cells in coupled single-cell TCR-pMHC assay

2025-12-22

Abstract excerpt

Determining T-cell specificity forms a crucial step toward understanding T-cell involvement in health and disease. Single-cell sequencing technologies allow for co-capture of TCR alpha and beta chains, and their antigen specificity can be determined through peptide-MHC (pMHC) multimer binding and capture of a co-attached barcode oligo. However, SC sequencing often includes a high level of dropouts and risk of cros...

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Literature Corpus work: 0147757b-0851-5598-b0d7-1536cc3edb9b
DOI: 10.64898/2025.12.19.695506

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