Article

A CRISPR-induced DNA break can trigger crossover, chromosomal loss and chromothripsis-like rearrangements

2023-05-24

Abstract excerpt

The fate of DNA double-strand breaks (DSBs) generated by the Cas9 nuclease has been thoroughly studied. Repair via non-homologous end-joining (NHEJ) or homologous recombination (HR) is the common outcome. However, little is known about unrepaired DSBs and the type of damage they can trigger in plants. In this work, we designed a new assay that detects loss of heterozygosity (LOH) in somatic cells, enabling the stu...

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Literature Corpus work: 00f3c63a-e5f4-5366-b615-0d38540191f9
DOI: 10.1101/2023.05.22.541757

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