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Article

CRISPR-Cas9-mediated chromosome engineering in Arabidopsis thaliana.

Nature protocols - 1 May 2022

Rönspies Michelle, Schindele Patrick, Wetzel Rebecca, Puchta Holger

Abstract excerpt

The rise of the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system has made it possible to induce double-strand breaks at almost any desired target site in the genome. In plant somatic cells, double-strand breaks are predominantly repaired by the error-prone nonhomologous end-joining pathway, which can lead to mutations at the break site upon repair. So far,...

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PMID: 35388178
DOI: 10.1038/s41596-022-00686-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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