CRISPR-Cas9-mediated chromosome engineering in Arabidopsis thaliana.

Abstract excerpt

The rise of the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system has made it possible to induce double-strand breaks at almost any desired target site in the genome. In plant somatic cells, double-strand breaks are predominantly repaired by the error-prone nonhomologous end-joining pathway, which can lead to mutations at the break site upon repair. So far,...

Topics

• Arabidopsis
• CRISPR-Cas Systems
• Chromosomes
• Gene Editing
• Mutation
• RNA, Guide, CRISPR-Cas Systems