Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae.

Nature communications - 22 Nov 2022

Huang Jun, Rowe David, Subedi Pratima, Zhang Wei, Suelter Tyler, Valent Barbara, Cook David E

Abstract excerpt

CRISPR-Cas mediated genome engineering has revolutionized functional genomics. However, understanding of DNA repair following Cas-mediated DNA cleavage remains incomplete. Using Cas12a ribonucleoprotein genome editing in the fungal pathogen, Magnaporthe oryzae, we detail non-canonical DNA repair outcomes from hundreds of transformants. Sanger and nanopore sequencing analysis reveals significant variation in DNA...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Ascomycota
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Breaks, Double-Stranded

Share to Mutation

Source

Open in PubMed Open full text

Identifiers

PMID: 36418866
DOI: 10.1038/s41467-022-34736-1
PMCID: PMC9684475

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae.PMID 36418866Read ↗

The contribution of DNA repair pathways to genome editing and evolution in filamentous pathogens.Semantic proximity Read ↗

Advances in CRISPR Plant ApplicationsSemantic proximity Read ↗

Development of genome-editing technologies for plant pathogenic fungiSemantic proximity Read ↗

Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9.Semantic proximity Read ↗

Altered DNA repair pathway engagement by engineered CRISPR-Cas9 nucleases.Semantic proximity Read ↗

Genetic Engineering in Bacteria, Fungi, and OomycetesSemantic proximity Read ↗

Improved genome editing by an engineered CRISPR-Cas12aSemantic proximity Read ↗

CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis.Semantic proximity Read ↗

Developmental progression of DNA double-strand break repair deciphered by a single-allele resolution mutation classifier.Semantic proximity Read ↗

A mutational scar-based genome-wide map of DNA double-strand break repair.Semantic proximity Read ↗

Mutational signature catalogue <i>(MUSIC)</i> of DNA double-strand break repairSemantic proximity Read ↗

Patterns of CRISPR/Cas9 activity in plants, animals and microbes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.