Spike-in–normalised single-cell RNA-seq reveals cell-type-specific transcriptional repression during ageing

Abstract excerpt

Transcriptome analyses are widely used for biomarker discovery and to gain insights into normal processes and diseases. Age-related changes in gene expression inferred from RNA-seq are typically reported relative to the transcriptome composition using library-size normalisation. As such, absolute changes in transcript abundance with age remain poorly characterised. Here, using external spike-in normalisation in th...

Topics

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• Adipose Tissue and Metabolism
• CD4-Positive T-Lymphocytes
• Cellular Senescence
• Immunologic Memory
• Lymphocyte Activation
• Molecular Biology Techniques and Applications
• Receptors, Antigen, T-Cell
• Single-cell and spatial transcriptomics

Identifiers and source

Literature Corpus work

00d7ca5e-d4f1-5066-acbf-3a7a4e733fba

DOI

10.64898/2026.06.25.733584