Article

Site-specific integration of transposon via engineered piggyBac transposase

2026-01-21

Abstract excerpt

The precise integration of large DNA fragments into the human genome holds significant therapeutic potential. Here, we demonstrate that combining engineered piggyBac (PB) transposase with CRISPR/Cas9 enables targeted integration of PB transposons into specified genomic loci. Our engineered PB transposase (PBase) retains high excision activity while substantially reducing endogenous integration activity. In the dev...

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Literature Corpus work: 006fccca-ef4e-5e9c-9a96-7d84ed543530
DOI: 10.64898/2026.01.20.700476

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