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Article

An efficient and reversible transposable system for gene delivery and lineage-specific differentiation in human embryonic stem cells.

Cell stem cell - 4 Sept 2009

Lacoste Arnaud, Berenshteyn Frada, Brivanlou Ali H

Abstract excerpt

Unraveling the therapeutic potential of human embryonic stem cells (hESC) requires tools to modify their genome. We have engineered the PiggyBac transposable element to create an efficient system for gene delivery in hESCs. This redesigned system, named "ePiggyBac," can deliver up to 18 Kb inserts, and transgene expression is observed in almost 90% of hES cells. ePiggyBac transposons can also carry insulators,...

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Identifiers

PMID: 19733544
DOI: 10.1016/j.stem.2009.07.011

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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