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Article

piggyBac-mediated genomic integration of linear dsDNA-based library for deep mutational scanning in mammalian cells.

Cellular and molecular life sciences : CMLS - 10 Oct 2023

Wang Yi, Zhao Yanjie, Li Yifan, Zhang Kaili, Fan Yan, Li Bo, Su Weijun, Li Shuai

Abstract excerpt

Deep mutational scanning (DMS) makes it possible to perform massively parallel quantification of the relationship between genetic variants and phenotypes of interest. However, the difficulties in introducing large variant libraries into mammalian cells greatly hinder DMS under physiological states. Here, we developed two novel strategies for DMS library construction in mammalian cells, namely 'piggyBac-in vitro...

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PMID: 37815732
DOI: 10.1007/s00018-023-04976-5
PMCID: PMC11071730

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