Single-tube genotyping without oligonucleotide probes.

Article

Single-tube genotyping without oligonucleotide probes.

Genome research - 1 Jan 1999

Germer S, Higuchi R

Abstract excerpt

We report the development of a self-contained (homogeneous), single-tube assay for the genotyping of single-nucleotide polymorphisms (SNPs), which does not rely on fluorescent oligonucleotide probes. The method, which we call Tm-shift genotyping, combines allele-specific PCR with the discriminati...

Topics

Genotype
Humans
Oligonucleotide Probes
Polymerase Chain Reaction
Polymorphism, Genetic
Reproducibility of Results
Sequence Analysis, DNA

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