Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Single-tube genotyping without oligonucleotide probes.

Genome research - 1 Jan 1999

Germer S, Higuchi R

Abstract excerpt

We report the development of a self-contained (homogeneous), single-tube assay for the genotyping of single-nucleotide polymorphisms (SNPs), which does not rely on fluorescent oligonucleotide probes. The method, which we call Tm-shift genotyping, combines allele-specific PCR with the discrimination between amplification products by their melting temperatures (Tm). Two distinct forward primers, each of which...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genotype
Share to Oligonucleotide Probes
Share to Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Polymorphism, Genetic

Share to Reproducibility of Results

Share to Sequence Analysis, DNA

Source

Open in PubMed Open full text

Identifiers

PMID: 9927486
PMCID: PMC310703

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Single-tube genotyping without oligonucleotide probes.PMID 9927486Read ↗

High-throughput SNP genotyping by single-tube PCR with Tm-shift primers.Semantic proximity Read ↗

Miniaturized sealed-tube allele-specific PCR.Semantic proximity Read ↗

A homogeneous fluorescence assay for PCR amplicons: its application to real-time, single-tube genotyping.Semantic proximity Read ↗

SNP genotyping using single-tube fluorescent bidirectional PCR.Semantic proximity Read ↗

High-resolution melting analysis of single nucleotide polymorphisms.Semantic proximity Read ↗

Novel and alternate SNP and genetic technologies.Semantic proximity Read ↗

Competitive SNP-LAMP probes for rapid and robust single-nucleotide polymorphism detectionSemantic proximity Read ↗

[Application of Tm-shift genotyping method in genetic studies].Semantic proximity Read ↗

The LightTyper: high-throughput genotyping using fluorescent melting curve analysis.Semantic proximity Read ↗

Single nucleotide polymorphism genotyping using allele-specific PCR and fluorescence melting curves.Semantic proximity Read ↗

Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons.Semantic proximity Read ↗

Detection of single-nucleotide polymorphisms using gold nanoparticles and single-strand-specific nucleases.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.