Article
[Application of Tm-shift genotyping method in genetic studies].
Yi chuan = Hereditas - 1 Nov 2012
Yuan Fang, Xu Jin, Ji Lin-Dan, Fei Li-Juan, Liu Pan-Pan, Zhang Li-Na
Abstract excerpt
Melting Temperature shift (Tm-shift) is a new genotyping method. With two GC-rich tails of unequal length combined to 5'-terminal of allele-specific primers, genotypes can be determined by the distinct Tms of the PCR products with inspection of a melting curve on the real-time PCR machine. In this study, 29 SNPs were genotyped with 2 048 samples by using Tm-shift genotyping method, and the results were assessed...
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