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Article

Single nucleotide polymorphism genotyping using allele-specific PCR and fluorescence melting curves.

BioTechniques - 1 May 2003

Papp Audrey C, Pinsonneault Julia K, Cooke Glen, Sadée Wolfgang

Abstract excerpt

We present a PCR method for identification of single nucleotide polymorphisms (SNPs), using allele-specific primers designed for selective amplification of each allele. Matching the SNP at the 3' end of the forward or reverse primer, and additionally incorporating a 3' mismatch to prevent amplifi...

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PMID: 12765033
DOI: 10.2144/03345dd03

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ABCA1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-12765033-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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