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Article

A homogeneous fluorescence assay for PCR amplicons: its application to real-time, single-tube genotyping.

Clinical chemistry - 1 May 1998

Whitcombe D, Brownie J, Gillard H L, McKechnie D, Theaker J, Newton C R, Little S

Abstract excerpt

We have developed a method whereby a single TaqMan probe can be used for many PCR reactions. We demonstrate its application as an integrated system for the direct measurement of allele-specific amplicon generation coupled to the suppression of primer-dimer accumulation in PCR. The system uses a 5'-exonuclease assay of amplicon annealed fluorogenic probes that operates in conjunction with the Amplification...

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PMID: 9590362

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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