A homogeneous fluorescence assay for PCR amplicons: its application to real-time, single-tube genotyping.

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Article

A homogeneous fluorescence assay for PCR amplicons: its application to real-time, single-tube genotyping.

Clinical chemistry - 1 May 1998

Whitcombe D, Brownie J, Gillard H L, McKechnie D, Theaker J, Newton C R, Little S

Abstract excerpt

We have developed a method whereby a single TaqMan probe can be used for many PCR reactions. We demonstrate its application as an integrated system for the direct measurement of allele-specific amplicon generation coupled to the suppression of primer-dimer accumulation in PCR. The system uses a 5...

Topics

Alleles
BRCA2 Protein
Breast Neoplasms
Cystic Fibrosis
DNA
DNA, Neoplasm
Factor V
Female
Fluoresceins
Fluorescent Dyes
Genotype
Humans
Neoplasm Proteins
Oligonucleotide Probes
Polymerase Chain Reaction
Polymorphism, Genetic
Sequence Analysis, DNA
Transcription Factors

Source

Open in PubMed

Identifiers

PMID: 9590362

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9590362-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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