Article
The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression.
Proceedings of the National Academy of Sciences of the United States of America - 19 Jan 1999
Balciunas D, Gälman C, Ronne H, Björklund S
Abstract excerpt
The mediator complex is essential for regulated transcription in vitro. In the yeast Saccharomyces cerevisiae, mediator comprises >15 subunits and interacts with the C-terminal domain of the largest subunit of RNA polymerase II, thus forming an RNA polymerase II holoenzyme. Here we describe the m...
Topics
- Cloning, Molecular
- Cyclin-Dependent Kinases
- Cyclins
- Fungal Proteins
- Gene Deletion
- Gene Expression Regulation, Fungal
- Phenotype
- Protein Serine-Threonine Kinases
- RNA Polymerase II
- Recombinant Proteins
- Repressor Proteins
- Saccharomyces cerevisiae
- Trans-Activators
- Transcription, Genetic
- Transcriptional Activation