Article
A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans.
Molecular and cellular biology - 1 Aug 1997
Lee Y C, Min S, Gim B S, Kim Y J
Abstract excerpt
A temperature-sensitive mutation was obtained in Med6p, a component of the mediator complex from the yeast Saccharomyces cerevisiae. The mediator complex has been shown to enable transcriptional activation in vitro. This mutation in Med6p abolished activation of transcription from four of five inducible promoters tested in vivo. There was no effect, however, on uninduced transcription, transcription of...
Topics
- Amino Acid Sequence
- Carrier Proteins
- Cell Extracts
- Cell Nucleus
- Cloning, Molecular
- Coenzymes
- DNA-Directed RNA Polymerases
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Humans
- Mating Factor
- Mediator Complex