Detection of HLA-B27 alleles by group-specific amplification and time-resolved fluorometry.

doi:10.1016/s0022-1759(98)00137-9

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Article

Detection of HLA-B27 alleles by group-specific amplification and time-resolved fluorometry.

Journal of immunological methods - 1 Oct 1998

Välimaa L, Sjöroos M, Luhtala M, Toivanen P, Lövgren T, Ilonen J

Abstract excerpt

This newly developed HLA-B27 assay combines a polymerase chain reaction (PCR) from blood spot samples with solution hybridisation in microtitration plate and with time-resolved fluorometry (TRF) as the detection system. In a multiplex amplification reaction, the 144 base pair region of HLA-B27 al...

Topics

Alleles
False Negative Reactions
Fluorometry
HLA-B27 Antigen
Heparin
Humans
Nucleic Acid Hybridization
Oligonucleotide Probes
Polymerase Chain Reaction
Sensitivity and Specificity
Streptavidin
Time Factors

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