Article
A homogeneous HLA-B*27 genotyping assay using dried reagent mixtures.
Disease markers - 1 Jan 2009
Kiviniemi Minna, Ilonen Jorma, Lövgren Timo
Abstract excerpt
The presence of HLA-B*27 allele with patients suspected with ankylosing spondylitis can be used in the diagnostic process. We have developed an assay for typing for the HLA-B*27 in whole blood dried on sample collection cards using pre-dried reagent wells and homogeneous time-resolved fluorescence based PCR approach. Essentially only the sample needs to be added to the dry ready-to-use reaction well in order to...
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