Article
Comparison of two HLA-DRB high resolution microtiter plate reverse hybridization typing methods: advantage of a codon-86 valine or glycine PCR segregation.
Tissue antigens - 1 Feb 1995
Peponnet C, Schaeffer V, Lepage V, Chatelain F, Rodde I, Alsayed J, Boucher P, Hermans P, Monplaisir/Cassius de Linval N, Charron D
Abstract excerpt
Two rapid, nonisotopic, high-resolution HLA-DRB typing methods have been developed for DRB1, DRB3, DRB4 and DRB5 alleles. These methods are based on a single procedure consisting of the reverse hybridization of biotinylated amplicons to oligonucleotide probes that are covalently attached to a mic...
Topics
- Alleles
- Base Sequence
- Codon
- Genes, MHC Class II
- Genotype
- Glycine
- HLA-DR Antigens
- HLA-DRB1 Chains
- HLA-DRB3 Chains
- HLA-DRB4 Chains
- HLA-DRB5 Chains
- Histocompatibility Testing
- Humans
- Microchemistry