Article
Comparison of two HLA-DRB high resolution microtiter plate reverse hybridization typing methods: advantage of a codon-86 valine or glycine PCR segregation.
Tissue antigens - 1 Feb 1995
Peponnet C, Schaeffer V, Lepage V, Chatelain F, Rodde I, Alsayed J, Boucher P, Hermans P, Monplaisir/Cassius de Linval N, Charron D
Abstract excerpt
Two rapid, nonisotopic, high-resolution HLA-DRB typing methods have been developed for DRB1, DRB3, DRB4 and DRB5 alleles. These methods are based on a single procedure consisting of the reverse hybridization of biotinylated amplicons to oligonucleotide probes that are covalently attached to a microtiter plate. Detection is by an enzymatic reaction with a fluorescent substrate. The 1 Generic Amplification (1GA)...
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