Article
Triple-label hybridization assay for type-1 diabetes-related HLA alleles.
BioTechniques - 1 May 1995
Sjöroos M, Iitiä A, Ilonen J, Reijonen H, Lövgren T
Abstract excerpt
We describe a method for the detection of two type 1 (insulin-dependent) diabetes susceptibility (*0201, *0302) alleles and two protective (*0301, *0602/0603) alleles of the HLA-DQB1 gene on the human major histocompatibility complex (MHC). The test is based on DNA amplification with PCR followed by simultaneous, allele-specific triple-label hybridization performed in microtitration wells. In the hybridization,...
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