Article
HLA-B27 genotyping by fluorescent resonance emission transfer (FRET) probes in real-time PCR.
Human immunology - 1 Aug 2004
Faner Rosa, Casamitjana Natàlia, Colobran Roger, Ribera Anna, Pujol-Borrell Ricardo, Palou Eduard, Juan Manel
Abstract excerpt
Several polymerase chain reaction (PCR)-based human leukocyte antigen (HLA) genotyping methods are in use, but none is fully satisfactory. The introduction of real-time PCR (rt-PCR) with fluorescence resonance energy transfer (FRET) probes provides a powerful tool to overcome the drawbacks of current methods such as the long processing time and the requirement for post-PCR manual procedures. Here we present...
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