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Article

Real-time PCR using fluorescent resonance emission transfer probes for HLA-B typing.

Human immunology - 1 Jan 2000

Faner Rosa, Casamitjana Natàlia, Coll Jordi, Caro Pepi, Pujol-Borrell Ricardo, Palou Eduard, Juan Manel

Abstract excerpt

HLA genotyping by polymerase chain reaction (PCR) has some inherent labor-intensive and effort-demanding limitations. To overcome them, we have developed a real-time PCR with hybridization probes approach able to obtain a medium-low resolution HLA-B genotyping with fewer tubes and probes and with a shorter time requirement. Our strategy used 18 simultaneous reactions amplifying HLA-B alleles and an internal...

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PMID: 16720220
DOI: 10.1016/j.humimm.2006.02.038

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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