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Article

Time-resolved fluorometry based sandwich hybridisation assay for HLA-DQA1 typing.

Disease markers - 1 Jun 1998

Sjöroos M, Ilonen J, Reijonen H, Lövgren T

Abstract excerpt

A microtitration plate based time-resolved fluorescence (TRF) hybridisation assay was developed for HLA typing utilising biotinylated sequence-specific catching probes and europium (Eu) labelled gene locus-specific detection probe to allow time-resolved fluorometer reading of the reaction. In an application for HLA-DQA typing a 228 base pair long region of the polymorphic exon 2 of DQA1 gene was amplified and the...

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Identifiers

PMID: 9706458
DOI: 10.1155/1998/350145
PMCID: PMC3851660

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