Article
Time-resolved fluorometry based sandwich hybridisation assay for HLA-DQA1 typing.
Disease markers - 1 Jun 1998
Sjöroos M, Ilonen J, Reijonen H, Lövgren T
Abstract excerpt
A microtitration plate based time-resolved fluorescence (TRF) hybridisation assay was developed for HLA typing utilising biotinylated sequence-specific catching probes and europium (Eu) labelled gene locus-specific detection probe to allow time-resolved fluorometer reading of the reaction. In an...
Topics
- DNA Primers
- Fluorometry
- Genotype
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- Humans
- Nucleic Acid Hybridization
- Polymerase Chain Reaction