Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Luminex-Based Methods in High-Resolution HLA Typing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Testi Manuela, Andreani Marco

Abstract excerpt

Luminex-based technology has been applied to discriminate between the different Human Leukocyte Antigens (HLA) alleles. The typing method consists in a reverse-SSO assay: Target DNA is PCR-amplified using biotinylated group-specific primers. A single PCR reaction is used for each HLA locus. The biotinylated PCR product is chemically denatured using a pH change and allowed to rehybridize to complementary DNA...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biotinylation
Share to DNA
Share to DNA Primers

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Fluorescent Dyes

Share to Genotyping Techniques

Share to Histocompatibility Testing

Share to HLA Antigens

Share to Nucleic Acid Hybridization

Source

Identifiers

PMID: 26024639
DOI: 10.1007/978-1-4939-2690-9_19

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Luminex-Based Methods in High-Resolution HLA Typing.PMID 26024639Read ↗

HLA typing using bead-based methods.Semantic proximity Read ↗

HLA Genotyping and Antibody Characterization using the Luminex<sup>TM</sup> Multiplex TechnologySemantic proximity Read ↗

Monitoring native HLA-I trimer specific antibodies in Luminex multiplex single antigen bead assay: Evaluation of beadsets from different manufacturersSemantic proximity Read ↗

Low-medium resolution HLA-DQ2/DQ8 typing for coeliac disease predisposition analysis by colorimetric assay.Semantic proximity Read ↗

Novel Approaches and Technologies in Molecular HLA Typing.Semantic proximity Read ↗

Combined flash- and glow-type chemiluminescent reactions for high-throughput genotyping of biallelic polymorphisms.Semantic proximity Read ↗

HLA techniques: Typing and antibody detection in the laboratory of immunogeneticsSemantic proximity Read ↗

Multiplex single nucleotide extension: a robust and high throughput method for HLA-A locus typing.Semantic proximity Read ↗

Deciphering the role of the conjugate's phycoerythrin label in complement-mediated interference occurring in HLA single antigen Luminex bead assays.Semantic proximity Read ↗

High-throughput DNA typing of HLA-A, -B, -C, and -DRB1 loci by a PCR-SSOP-Luminex method in the Japanese population.Semantic proximity Read ↗

A combination of two distinct in vitro amplification procedures for DNA typing of HLA-DRB and -DQB 1 alleles.Semantic proximity Read ↗

An overview of the Progenika ID CORE XT: an automated genotyping platform based on a fluidic microarray system.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.