Article
Rapid screening for HLA-B27 by a TaqMan-PCR assay using sequence-specific primers and a minor groove binder probe, a novel type of TaqMan trade mark probe.
Journal of immunological methods - 1 Apr 2004
Kuzio Sylvain, Sylvain Kuzio, Hanguehard Aurélie, Aurélie Hanguehard, Morelle Marc, Marc Morelle, Ronsin Christophe, Christophe Ronsin
Abstract excerpt
HLA-B27 is strongly associated with ankylosing spondylitis (AS). As typing for HLA-B27 is routinely performed by serological methods, false-positive results can be generated. Therefore, several more accurate molecular methods have been developed for HLA-B27 genotyping. We describe a real-time PCR...
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